Which change would have little impact on the safety and effectiveness of an application during operations?

Moving the operation to a different mixing-loading location would have little impact on the safety and effectiveness of an aerial application. This is because the primary factors influencing the safety and effectiveness of pesticide application are related to the handling of the chemicals, including proper mixing and loading techniques, adherence to safety protocols, and ensuring accurate application rates.

The safety of the application is largely determined by how well the operator follows safety guidelines during the mixing and loading process, irrespective of the specific location. As long as the new location supports proper mixing and loading practices (such as being equipped with necessary safety equipment and containment systems), it won't significantly alter the outcomes of the application in terms of efficacy or safety.

In contrast, changing the application site, altering the chemical mixture, or scheduling different flight times can significantly impact both effectiveness and safety due to potential variations in environmental conditions, the properties of the chemicals being used, and the timing of the application relative to target pest activity and environmental factors.